Review



rabbit anti human ve cadherin  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rabbit anti human ve cadherin
    Rabbit Anti Human Ve Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/pm41944190-237-32-35
    Average 86 stars, based on 1 article reviews
    rabbit anti human ve cadherin - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    other:

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model
    Article Snippet: Rabbit anti-VE-cadherin , Cell Signaling , Cat #D87F2.

    Article Title: Protocol for generation of endothelial cells from human-derived iPSCs and integration into mouse brain explants
    Article Snippet: Rabbit anti-VE-cadherin (1:100 dilution) , Cell Signaling Technology , 2500; RRID: AB_10839118.

    Infection:

    Article Title: Trypanosoma cruzi infection-induced changes in cardiac microvascular endothelial cell morphology and function.
    Article Snippet: .. At days 1 and 5 post- infection (p.i.), monolayers were fixed in 4% paraformaldehyde (Electron Microscopy Sciences Cat. 15710) for 20 min, permeabilized for 5 min with 0.1% Triton X- 100 (Sigma- Aldrich Cat. T9284) and blocked with immunofluorescence blocking buffer (Cell Signaling Technology Cat. 12411) for 1 h. Coverslips were then stained with rabbit anti- VE- cadherin (VE- CAD) (Cell Signaling Technology Cat. 2500) diluted in 1:500 PBS (Sigma Cat. P3563)+0.05% Tween 20 (Sigma- Aldrich Cat. P1379) overnight at 4 °C with rocking. .. After overnight incubation, coverslips were stained with anti- rabbit F(ab')2 Fragment (Alexa Fluor 488 Conjugate) diluted 1:1,000 (Cell Signaling Technologies Cat. 4412) in PBS+0.05% Tween 20 for 2 h at room temperature with rocking.

    Electron Microscopy:

    Article Title: Trypanosoma cruzi infection-induced changes in cardiac microvascular endothelial cell morphology and function.
    Article Snippet: .. At days 1 and 5 post- infection (p.i.), monolayers were fixed in 4% paraformaldehyde (Electron Microscopy Sciences Cat. 15710) for 20 min, permeabilized for 5 min with 0.1% Triton X- 100 (Sigma- Aldrich Cat. T9284) and blocked with immunofluorescence blocking buffer (Cell Signaling Technology Cat. 12411) for 1 h. Coverslips were then stained with rabbit anti- VE- cadherin (VE- CAD) (Cell Signaling Technology Cat. 2500) diluted in 1:500 PBS (Sigma Cat. P3563)+0.05% Tween 20 (Sigma- Aldrich Cat. P1379) overnight at 4 °C with rocking. .. After overnight incubation, coverslips were stained with anti- rabbit F(ab')2 Fragment (Alexa Fluor 488 Conjugate) diluted 1:1,000 (Cell Signaling Technologies Cat. 4412) in PBS+0.05% Tween 20 for 2 h at room temperature with rocking.

    Immunofluorescence:

    Article Title: Trypanosoma cruzi infection-induced changes in cardiac microvascular endothelial cell morphology and function.
    Article Snippet: .. At days 1 and 5 post- infection (p.i.), monolayers were fixed in 4% paraformaldehyde (Electron Microscopy Sciences Cat. 15710) for 20 min, permeabilized for 5 min with 0.1% Triton X- 100 (Sigma- Aldrich Cat. T9284) and blocked with immunofluorescence blocking buffer (Cell Signaling Technology Cat. 12411) for 1 h. Coverslips were then stained with rabbit anti- VE- cadherin (VE- CAD) (Cell Signaling Technology Cat. 2500) diluted in 1:500 PBS (Sigma Cat. P3563)+0.05% Tween 20 (Sigma- Aldrich Cat. P1379) overnight at 4 °C with rocking. .. After overnight incubation, coverslips were stained with anti- rabbit F(ab')2 Fragment (Alexa Fluor 488 Conjugate) diluted 1:1,000 (Cell Signaling Technologies Cat. 4412) in PBS+0.05% Tween 20 for 2 h at room temperature with rocking.

    Blocking Assay:

    Article Title: Trypanosoma cruzi infection-induced changes in cardiac microvascular endothelial cell morphology and function.
    Article Snippet: .. At days 1 and 5 post- infection (p.i.), monolayers were fixed in 4% paraformaldehyde (Electron Microscopy Sciences Cat. 15710) for 20 min, permeabilized for 5 min with 0.1% Triton X- 100 (Sigma- Aldrich Cat. T9284) and blocked with immunofluorescence blocking buffer (Cell Signaling Technology Cat. 12411) for 1 h. Coverslips were then stained with rabbit anti- VE- cadherin (VE- CAD) (Cell Signaling Technology Cat. 2500) diluted in 1:500 PBS (Sigma Cat. P3563)+0.05% Tween 20 (Sigma- Aldrich Cat. P1379) overnight at 4 °C with rocking. .. After overnight incubation, coverslips were stained with anti- rabbit F(ab')2 Fragment (Alexa Fluor 488 Conjugate) diluted 1:1,000 (Cell Signaling Technologies Cat. 4412) in PBS+0.05% Tween 20 for 2 h at room temperature with rocking.

    Staining:

    Article Title: Trypanosoma cruzi infection-induced changes in cardiac microvascular endothelial cell morphology and function.
    Article Snippet: .. At days 1 and 5 post- infection (p.i.), monolayers were fixed in 4% paraformaldehyde (Electron Microscopy Sciences Cat. 15710) for 20 min, permeabilized for 5 min with 0.1% Triton X- 100 (Sigma- Aldrich Cat. T9284) and blocked with immunofluorescence blocking buffer (Cell Signaling Technology Cat. 12411) for 1 h. Coverslips were then stained with rabbit anti- VE- cadherin (VE- CAD) (Cell Signaling Technology Cat. 2500) diluted in 1:500 PBS (Sigma Cat. P3563)+0.05% Tween 20 (Sigma- Aldrich Cat. P1379) overnight at 4 °C with rocking. .. After overnight incubation, coverslips were stained with anti- rabbit F(ab')2 Fragment (Alexa Fluor 488 Conjugate) diluted 1:1,000 (Cell Signaling Technologies Cat. 4412) in PBS+0.05% Tween 20 for 2 h at room temperature with rocking.

    Incubation:

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model
    Article Snippet: Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20 mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anti-claudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS) + 0.2% Tween-20 at 4 °C, overnight on a shaker. .. After three 5 min TBS + 0.2% Tween-20 washes, membranes were incubated with 1:10,000 IRDye goat anti-mouse 680RD (926-68070, LI-COR Biotech) and 1:20,000 IRDye goat anti-rabbit 800CW (926-32211, LI-COR Biotech) in 3% BSA-TBS + 0.2% Tween-20 + 0.01% sodium dodecyl sulphate for 1 h at RT on a shaker.

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model.
    Article Snippet: Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anticlaudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS)+ 0.2% Tween-20 at 4 °C, overnight on a shaker. .. After three 5min TBS+ 0.2% Tween-20 washes, membranes were incubated with 1:10,000 IRDye goat antimouse 680RD (926-68070, LI-COR Biotech) and 1:20,000 IRDye goat anti-rabbit 800CW (926-32211, LI-COR Biotech) in 3% BSA-TBS+ 0.2% Tween-20+ 0.01% sodium dodecyl sulphate for 1 h at RT on a shaker.

    Article Title: HSP70 governs permeability and mechanotransduction in primary human endothelial cells.
    Article Snippet: .. Cells were incubated overnight at 4 °C with the following primary antibodies diluted in 1% BSA buffer: rabbit anti-VE-cadherin (1 : 400; Abcam, ab33168) or rabbit anti-VE-cadherin (1 : 400; Cell Signaling Technology, 2500) and mouse anti-PECAM-1 (1 : 200; Cell Signaling Technology, 3528S). .. After several washes, cells were incubated for 1 h at room temperature with the appropriate secondary antibodies: Alexa Fluor 488-conjugated goat anti-rabbit IgG and Alexa Fluor 546-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, Waltham, MA, USA), both diluted 1 : 400.

    Saline:

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model
    Article Snippet: Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20 mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anti-claudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS) + 0.2% Tween-20 at 4 °C, overnight on a shaker. .. After three 5 min TBS + 0.2% Tween-20 washes, membranes were incubated with 1:10,000 IRDye goat anti-mouse 680RD (926-68070, LI-COR Biotech) and 1:20,000 IRDye goat anti-rabbit 800CW (926-32211, LI-COR Biotech) in 3% BSA-TBS + 0.2% Tween-20 + 0.01% sodium dodecyl sulphate for 1 h at RT on a shaker.

    Article Title: Plasmodium falciparum impairs Ang-1 secretion by pericytes in a 3D brain microvessel model.
    Article Snippet: Protein concentrations were quantified using a BCA assay (23227, Invitrogen) following the manufacturer's protocols, and 20mg of protein was ran on precast Mini-PROTEAN TGX gels (4561034, Bio-Rad), along with 5 μL of Odyssey One-Color Protein Molecular Weight Marker (928-40000, LI-COR Biotech) or Precision Plus Protein WesternC Blotting Standard (1610376, Bio-Rad) at 170 V for 1 h. Gels were transferred to nitrocellulose membranes (1704159, Bio-Rad) using a Trans-blot Turbo transfer machine set to mixed molecular weight (2.5 A, 25 V). .. Membranes were blocked in 5% BSA-TBS for 2 h on a shaker set to 50 rpm and then incubated with 1:1000 rabbit anticlaudin-5 (E8F3D, Cell signaling), 1:1000 rabbit anti-VE-cadherin (D87F2, Cell signaling) or 1:1000 mouse anti-β-tubulin (MA5-16308, Invitrogen) in 3% BSA-Tris-buffered saline (TBS)+ 0.2% Tween-20 at 4 °C, overnight on a shaker. .. After three 5min TBS+ 0.2% Tween-20 washes, membranes were incubated with 1:10,000 IRDye goat antimouse 680RD (926-68070, LI-COR Biotech) and 1:20,000 IRDye goat anti-rabbit 800CW (926-32211, LI-COR Biotech) in 3% BSA-TBS+ 0.2% Tween-20+ 0.01% sodium dodecyl sulphate for 1 h at RT on a shaker.



    Similar Products

    96
    Proteintech anti ve cadherin rabbit antibody
    Anti Ve Cadherin Rabbit Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/CDH5+Antibody/10__2147_slash_ijn__s618525-140-14-17
    Average 96 stars, based on 1 article reviews
    anti ve cadherin rabbit antibody - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    94
    ABclonal Biotechnology anti ve cadherin a0734
    Anti Ve Cadherin A0734, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/VE+Cadherin+Rabbit+pAb/pmc13525964-35-23-29
    Average 94 stars, based on 1 article reviews
    anti ve cadherin a0734 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    ABclonal Biotechnology ve cadherin
    Ve Cadherin, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/CDH5+Rabbit+pAb/pm42209461-78-0-27
    Average 94 stars, based on 1 article reviews
    ve cadherin - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    ABclonal Biotechnology a0734
    A0734, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/VE+Cadherin+Rabbit+pAb/us12616688-134-40-41
    Average 94 stars, based on 1 article reviews
    a0734 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    ABclonal Biotechnology anti ve cadherin antibody
    Identification of AVFS-Associated Cell Subpopulations and Candidate Genes. (A) UMAP plot showing the grouping of 10 cell types. (B) Expression of key marker genes across cell types. (C) Differential proportions of each cell type between AVFS and non-stenotic control. (D) Volcano plot of DEGs in endothelial cells. (E-G) GO, KEGG and Reactome enrichment analysis illustrating predominant biological functions and pathways. (H) Flow cytometry sorting and analysis of endothelial cells from vascular tissues, n = 3. (I) qRT-PCR analysis <t>of</t> <t>PPIB,</t> CD31, <t>VE-cadherin,</t> and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. (J) WB analysis of PPIB, CD31, VE-cadherin, and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. Data are presented as mean ± SD, differences between groups were analyzed by paired t-test. * P < 0.05; **<0.01; ***<0.001; ****<0.0001. SMC: Smooth muscle cell, EC: Endothelial cell, GO: Gene Ontology, KEGG: Kyoto Encyclopedia of Genes and Genomes.
    Anti Ve Cadherin Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/YB1+Rabbit+mAb/pmc13272917-211-14-16
    Average 94 stars, based on 1 article reviews
    anti ve cadherin antibody - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc rabbit anti human ve cadherin
    Identification of AVFS-Associated Cell Subpopulations and Candidate Genes. (A) UMAP plot showing the grouping of 10 cell types. (B) Expression of key marker genes across cell types. (C) Differential proportions of each cell type between AVFS and non-stenotic control. (D) Volcano plot of DEGs in endothelial cells. (E-G) GO, KEGG and Reactome enrichment analysis illustrating predominant biological functions and pathways. (H) Flow cytometry sorting and analysis of endothelial cells from vascular tissues, n = 3. (I) qRT-PCR analysis <t>of</t> <t>PPIB,</t> CD31, <t>VE-cadherin,</t> and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. (J) WB analysis of PPIB, CD31, VE-cadherin, and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. Data are presented as mean ± SD, differences between groups were analyzed by paired t-test. * P < 0.05; **<0.01; ***<0.001; ****<0.0001. SMC: Smooth muscle cell, EC: Endothelial cell, GO: Gene Ontology, KEGG: Kyoto Encyclopedia of Genes and Genomes.
    Rabbit Anti Human Ve Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/pm41944190-237-32-35
    Average 86 stars, based on 1 article reviews
    rabbit anti human ve cadherin - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc anti ve cadherin
    Identification of AVFS-Associated Cell Subpopulations and Candidate Genes. (A) UMAP plot showing the grouping of 10 cell types. (B) Expression of key marker genes across cell types. (C) Differential proportions of each cell type between AVFS and non-stenotic control. (D) Volcano plot of DEGs in endothelial cells. (E-G) GO, KEGG and Reactome enrichment analysis illustrating predominant biological functions and pathways. (H) Flow cytometry sorting and analysis of endothelial cells from vascular tissues, n = 3. (I) qRT-PCR analysis <t>of</t> <t>PPIB,</t> CD31, <t>VE-cadherin,</t> and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. (J) WB analysis of PPIB, CD31, VE-cadherin, and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. Data are presented as mean ± SD, differences between groups were analyzed by paired t-test. * P < 0.05; **<0.01; ***<0.001; ****<0.0001. SMC: Smooth muscle cell, EC: Endothelial cell, GO: Gene Ontology, KEGG: Kyoto Encyclopedia of Genes and Genomes.
    Anti Ve Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/VE-Cadherin+XP+Rabbit+mAb/pm41887219-989-65-68
    Average 96 stars, based on 1 article reviews
    anti ve cadherin - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc ve cadherin
    Identification of AVFS-Associated Cell Subpopulations and Candidate Genes. (A) UMAP plot showing the grouping of 10 cell types. (B) Expression of key marker genes across cell types. (C) Differential proportions of each cell type between AVFS and non-stenotic control. (D) Volcano plot of DEGs in endothelial cells. (E-G) GO, KEGG and Reactome enrichment analysis illustrating predominant biological functions and pathways. (H) Flow cytometry sorting and analysis of endothelial cells from vascular tissues, n = 3. (I) qRT-PCR analysis <t>of</t> <t>PPIB,</t> CD31, <t>VE-cadherin,</t> and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. (J) WB analysis of PPIB, CD31, VE-cadherin, and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. Data are presented as mean ± SD, differences between groups were analyzed by paired t-test. * P < 0.05; **<0.01; ***<0.001; ****<0.0001. SMC: Smooth muscle cell, EC: Endothelial cell, GO: Gene Ontology, KEGG: Kyoto Encyclopedia of Genes and Genomes.
    Ve Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ve+cadherin/VE-Cadherin+XP+Rabbit+mAb/pm41828479-238-22-25
    Average 96 stars, based on 1 article reviews
    ve cadherin - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Identification of AVFS-Associated Cell Subpopulations and Candidate Genes. (A) UMAP plot showing the grouping of 10 cell types. (B) Expression of key marker genes across cell types. (C) Differential proportions of each cell type between AVFS and non-stenotic control. (D) Volcano plot of DEGs in endothelial cells. (E-G) GO, KEGG and Reactome enrichment analysis illustrating predominant biological functions and pathways. (H) Flow cytometry sorting and analysis of endothelial cells from vascular tissues, n = 3. (I) qRT-PCR analysis of PPIB, CD31, VE-cadherin, and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. (J) WB analysis of PPIB, CD31, VE-cadherin, and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. Data are presented as mean ± SD, differences between groups were analyzed by paired t-test. * P < 0.05; **<0.01; ***<0.001; ****<0.0001. SMC: Smooth muscle cell, EC: Endothelial cell, GO: Gene Ontology, KEGG: Kyoto Encyclopedia of Genes and Genomes.

    Journal: Scientific Reports

    Article Title: YBX1/PPIB axis promotes post-maturation arteriovenous fistula stenosis via enhancing endothelial to mesenchymal transition

    doi: 10.1038/s41598-026-49568-y

    Figure Lengend Snippet: Identification of AVFS-Associated Cell Subpopulations and Candidate Genes. (A) UMAP plot showing the grouping of 10 cell types. (B) Expression of key marker genes across cell types. (C) Differential proportions of each cell type between AVFS and non-stenotic control. (D) Volcano plot of DEGs in endothelial cells. (E-G) GO, KEGG and Reactome enrichment analysis illustrating predominant biological functions and pathways. (H) Flow cytometry sorting and analysis of endothelial cells from vascular tissues, n = 3. (I) qRT-PCR analysis of PPIB, CD31, VE-cadherin, and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. (J) WB analysis of PPIB, CD31, VE-cadherin, and α-SMA expression levels in endothelial cells from AVFS and non-stenosis tissues, n = 3. Data are presented as mean ± SD, differences between groups were analyzed by paired t-test. * P < 0.05; **<0.01; ***<0.001; ****<0.0001. SMC: Smooth muscle cell, EC: Endothelial cell, GO: Gene Ontology, KEGG: Kyoto Encyclopedia of Genes and Genomes.

    Article Snippet: The antibodies used included: anti-PPIB antibody (CUSABIO, China; #CSB-PA018472ESR1HU), anti-YBX1 antibody (Abclonal, China; #A3534), anti-VE-cadherin antibody (Abclonal, China; #A12416), anti-CD31 antibody (Abclonal, China; #A19014), anti-α-SMA antibody (Affinity, China, #AF1032), and anti-GAPDH antibody (Abclonal, China; #A19056).

    Techniques: Expressing, Marker, Control, Flow Cytometry, Quantitative RT-PCR

    PPIB Regulates Endothelial Marker in HUVECs. (A) qRT-PCR of PPIB, VE-cadherin, and CD31 expression following PPIB knockdown in HUVECs ( n = 3). (B) qRT-PCR analysis of PPIB, VE-cadherin, and CD31 expression following PPIB overexpression in HUVECs ( n = 3). (C) Western blot of PPIB, VE-cadherin and CD31 after PPIB knockdown in HUVECs ( n = 3). (D) Western blot of PPIB, VE-cadherin, and CD31 expression after PPIB overexpression in HUVECs ( n = 3). The protein bands of WB were derived from different gels, and the uncropped original WB images are provided in the Supplementary Material 1. Data are presented as mean ± SD, differences between groups were analyzed by one-way ANOVA, statistical significance was determined using one-way ANOVA. Compared to the control group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; compared to the TGF-β1 group, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001. sh: short hairpin, gene expression interference by introducing short hairpin RNA. oe: overexpression, increasing gene expression by introducing the target gene sequence. NC: Negative control, for sh, refers to lentivirus construction by transfecting plasmids with a scrambled shRNA sequence; for oe, refers to lentivirus construction by directly transfecting empty plasmids. ns: not significant.

    Journal: Scientific Reports

    Article Title: YBX1/PPIB axis promotes post-maturation arteriovenous fistula stenosis via enhancing endothelial to mesenchymal transition

    doi: 10.1038/s41598-026-49568-y

    Figure Lengend Snippet: PPIB Regulates Endothelial Marker in HUVECs. (A) qRT-PCR of PPIB, VE-cadherin, and CD31 expression following PPIB knockdown in HUVECs ( n = 3). (B) qRT-PCR analysis of PPIB, VE-cadherin, and CD31 expression following PPIB overexpression in HUVECs ( n = 3). (C) Western blot of PPIB, VE-cadherin and CD31 after PPIB knockdown in HUVECs ( n = 3). (D) Western blot of PPIB, VE-cadherin, and CD31 expression after PPIB overexpression in HUVECs ( n = 3). The protein bands of WB were derived from different gels, and the uncropped original WB images are provided in the Supplementary Material 1. Data are presented as mean ± SD, differences between groups were analyzed by one-way ANOVA, statistical significance was determined using one-way ANOVA. Compared to the control group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; compared to the TGF-β1 group, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001. sh: short hairpin, gene expression interference by introducing short hairpin RNA. oe: overexpression, increasing gene expression by introducing the target gene sequence. NC: Negative control, for sh, refers to lentivirus construction by transfecting plasmids with a scrambled shRNA sequence; for oe, refers to lentivirus construction by directly transfecting empty plasmids. ns: not significant.

    Article Snippet: The antibodies used included: anti-PPIB antibody (CUSABIO, China; #CSB-PA018472ESR1HU), anti-YBX1 antibody (Abclonal, China; #A3534), anti-VE-cadherin antibody (Abclonal, China; #A12416), anti-CD31 antibody (Abclonal, China; #A19014), anti-α-SMA antibody (Affinity, China, #AF1032), and anti-GAPDH antibody (Abclonal, China; #A19056).

    Techniques: Marker, Quantitative RT-PCR, Expressing, Knockdown, Over Expression, Western Blot, Derivative Assay, Control, Gene Expression, shRNA, Sequencing, Negative Control

    Transcription Factor YBX1 is Associated with EndMT in Endothelial Cells. (A) Prediction of YBX1 binding sites in the PPIB promoter and schematic of site-directed mutations used in the dual-luciferase assay. (B) Dual-luciferase assay validating the interaction between YBX1 and the PPIB promoter region ( n = 3). (C) qRT-PCR analysis of YBX1, PPIB, VE-cadherin, and CD31 expression levels ( n = 3). (D–E) Western blot of YBX1, PPIB, VE-cadherin, and CD31 expression levels ( n = 3). (F , H) Immunofluorescence staining assessing α-SMA expression levels ( n = 3). (G , I) Wound healing assays evaluating HUVEC migration ability ( n = 3). The protein bands of WB were derived from different gels, and the uncropped original WB images are provided in the Supplementary Material 1. Data are presented as mean ± SD, differences between groups were analyzed by one-way ANOVA, statistical significance was determined using one-way ANOVA. * P < 0.05, **< 0.01, ***< 0.001, ****< 0.0001. luc: Firefly luciferase. RL: Renilla luciferase. WT: Wild type. Mut: Mutant. oe: overexpression, increasing gene expression by introducing the target gene sequence. NC: Negative control, for oe, refers to lentivirus construction by directly transfecting empty plasmids. ns: not significant.

    Journal: Scientific Reports

    Article Title: YBX1/PPIB axis promotes post-maturation arteriovenous fistula stenosis via enhancing endothelial to mesenchymal transition

    doi: 10.1038/s41598-026-49568-y

    Figure Lengend Snippet: Transcription Factor YBX1 is Associated with EndMT in Endothelial Cells. (A) Prediction of YBX1 binding sites in the PPIB promoter and schematic of site-directed mutations used in the dual-luciferase assay. (B) Dual-luciferase assay validating the interaction between YBX1 and the PPIB promoter region ( n = 3). (C) qRT-PCR analysis of YBX1, PPIB, VE-cadherin, and CD31 expression levels ( n = 3). (D–E) Western blot of YBX1, PPIB, VE-cadherin, and CD31 expression levels ( n = 3). (F , H) Immunofluorescence staining assessing α-SMA expression levels ( n = 3). (G , I) Wound healing assays evaluating HUVEC migration ability ( n = 3). The protein bands of WB were derived from different gels, and the uncropped original WB images are provided in the Supplementary Material 1. Data are presented as mean ± SD, differences between groups were analyzed by one-way ANOVA, statistical significance was determined using one-way ANOVA. * P < 0.05, **< 0.01, ***< 0.001, ****< 0.0001. luc: Firefly luciferase. RL: Renilla luciferase. WT: Wild type. Mut: Mutant. oe: overexpression, increasing gene expression by introducing the target gene sequence. NC: Negative control, for oe, refers to lentivirus construction by directly transfecting empty plasmids. ns: not significant.

    Article Snippet: The antibodies used included: anti-PPIB antibody (CUSABIO, China; #CSB-PA018472ESR1HU), anti-YBX1 antibody (Abclonal, China; #A3534), anti-VE-cadherin antibody (Abclonal, China; #A12416), anti-CD31 antibody (Abclonal, China; #A19014), anti-α-SMA antibody (Affinity, China, #AF1032), and anti-GAPDH antibody (Abclonal, China; #A19056).

    Techniques: Binding Assay, Luciferase, Quantitative RT-PCR, Expressing, Western Blot, Immunofluorescence, Staining, Migration, Derivative Assay, Mutagenesis, Over Expression, Gene Expression, Sequencing, Negative Control

    YBX1 Promotes EndMT through Regulation of PPIB. (A) qRT-PCR assessed the expression levels of YBX1, PPIB, VE-cadherin, and CD31 in each group, n = 3. (B) Western blot assessed the expression levels of YBX1, PPIB, VE-cadherin, and CD31 in each group, n = 3. (C) Immunofluorescence staining of α-SMA expression in HUVECs from each group ( n = 3). (D) Wound healing assay evaluating HUVEC migratory capacity in each group ( n = 3). The protein bands of WB were derived from different gels, and the uncropped original WB images are provided in the Supplementary Material 1. Data are presented as mean ± SD, differences between groups were analyzed by one-way ANOVA, statistical significance was determined using one-way ANOVA. * P < 0.05, **<0.01, ***<0.001, ****<0.0001 (vs. TGF-β1 group); # P < 0.05, ##<0.01, ###<0.001, ####<0.0001 (vs. YBX1-oe + NC-sh + TGF-β1 group); ^ P < 0.05, ^^<0.01, ^^^<0.001, ^^^^<0.0001 (vs. NC-oe+PPIB-sh + TGF-β1 group). sh: short hairpin, gene expression interference by introducing short hairpin RNA. oe: overexpression, increasing gene expression by introducing the target gene sequence. NC: Negative control, for sh, refers to lentivirus construction by transfecting plasmids with a scrambled shRNA sequence; for oe, refers to lentivirus construction by directly transfecting empty plasmids. ns: not significant.

    Journal: Scientific Reports

    Article Title: YBX1/PPIB axis promotes post-maturation arteriovenous fistula stenosis via enhancing endothelial to mesenchymal transition

    doi: 10.1038/s41598-026-49568-y

    Figure Lengend Snippet: YBX1 Promotes EndMT through Regulation of PPIB. (A) qRT-PCR assessed the expression levels of YBX1, PPIB, VE-cadherin, and CD31 in each group, n = 3. (B) Western blot assessed the expression levels of YBX1, PPIB, VE-cadherin, and CD31 in each group, n = 3. (C) Immunofluorescence staining of α-SMA expression in HUVECs from each group ( n = 3). (D) Wound healing assay evaluating HUVEC migratory capacity in each group ( n = 3). The protein bands of WB were derived from different gels, and the uncropped original WB images are provided in the Supplementary Material 1. Data are presented as mean ± SD, differences between groups were analyzed by one-way ANOVA, statistical significance was determined using one-way ANOVA. * P < 0.05, **<0.01, ***<0.001, ****<0.0001 (vs. TGF-β1 group); # P < 0.05, ##<0.01, ###<0.001, ####<0.0001 (vs. YBX1-oe + NC-sh + TGF-β1 group); ^ P < 0.05, ^^<0.01, ^^^<0.001, ^^^^<0.0001 (vs. NC-oe+PPIB-sh + TGF-β1 group). sh: short hairpin, gene expression interference by introducing short hairpin RNA. oe: overexpression, increasing gene expression by introducing the target gene sequence. NC: Negative control, for sh, refers to lentivirus construction by transfecting plasmids with a scrambled shRNA sequence; for oe, refers to lentivirus construction by directly transfecting empty plasmids. ns: not significant.

    Article Snippet: The antibodies used included: anti-PPIB antibody (CUSABIO, China; #CSB-PA018472ESR1HU), anti-YBX1 antibody (Abclonal, China; #A3534), anti-VE-cadherin antibody (Abclonal, China; #A12416), anti-CD31 antibody (Abclonal, China; #A19014), anti-α-SMA antibody (Affinity, China, #AF1032), and anti-GAPDH antibody (Abclonal, China; #A19056).

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunofluorescence, Staining, Wound Healing Assay, Derivative Assay, Gene Expression, shRNA, Over Expression, Sequencing, Negative Control